ISP Interface Cables
AVRISP Supports both the 6-pin header connector pinout, used by the Atmel AT90ISP cable, and the 10-pin header connector used by the STK200 and STK300 development boards. Figure 3-2 shows the pinouts for the 6-pin and 10-pin ISP connectors.
AVRISP is delivered with one 6-wire and one 10-wire ISP cable. Use the one that match the pinout of the target ISP connector. However, note that only one cable should be connected, and used, at any given time. By default the 10-pin header connector is mounted.